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Detection of Salmonella typhimurium and Escherichia coli in Contaminated Milk Sample using Real Time PCR Method
M Nurjayadi (a*), U R Efrianti (a), N Azizah (a), F Kurniadewi (a), V Saamia (b), I M Wiranatha (b), L Nastassya (c)

(a) Department of Chemistry, Mathematics and Science Faculty, Universitas Negeri Jakarta, Indonesia
(b) Pusat Laboratorium Forensik Badan Reserse Kriminal Kepolisian Republik Indonesia, Indonesia
(c) Balai Besar Uji Standar Karantina Pertanian, Jakarta, Indonesia


Abstract

Salmonella and Escherichia coli are the bacteria the most common cause of food-borne illness. The aim of the study was to detection S. typhimurium and E. coli by Real Time PCR from milk samples. Gene targeted of S. typhimurium was pef and the E. coli gene targeted was fimC. Confirmation test of all primers in contaminated milk with S. typhimurium and E. coli gave different cycle threshold (Ct) value in range 12-13. The detection limit for S. typhimurium with primer pef was in 7,92 pg/&

Keywords: Salmonella typhimurium, Escherichia coli, Milk Sample, Real Time PCR

Topic: Biology

Link: https://ifory.id/abstract/buMe4cWnKxAq

Conference: The 4th Annual Applied Science and Engineering Conference (AASEC 2019)

Plain Format | Corresponding Author (Muktiningsih Nurjayadi)

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