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S-Acyltransferase gene (pat) encodes a protein acyltransferase (PAT) acting as an enzyme catalyzing an acyl chain transfer to the molecular acceptor, mono- or di-acylglycerol, in the biosynthesis of triacylglycerol (TAG). So far, pat from tropical marine microalgae has yet been explored. We identified an microalgae isolate based on physical morphology and genetic marker of 18S rDNA. Finally, we characterized pat from reverse transcription of total RNA of the isolate, cloned the pat to pGemT and expressed in E. coli TOP10. The results showed that isolate obtained from Maluku water was green spherical in shape with a cell diameter of 2-10 µm. A 1644-bp nucleotide fragment of 18S rDNA was obtained and showed 99% nucleotide sequence similarity with that of Chlorella vulgaris (GenBank no. KF574391.1). The microalgae isolate was identified as Chlorella vulgaris CBI. Reverse transcription product from the total Chlorella vulgaris CBI RNA was a 600 bp nucleotide. The first 300 bp nucleotide was unidentified, but starting from nucleotide no. 301 to the end has 51% identity with pat fragment of Chlorella variabilis (GenBank no. XP_005849764.1). A 77-amino acid residues deduced was identify as the first N-terminus of PAT (domain transporter protein). This pat fragment of Chlorella vulgaris CBI was useful for starting point to find the whole pat seqeunce.