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Tangkue, one of rambutan (Nephelium lappaceum L) variety originated form Banten Province found to be mycotrophic. The aim of this study was to investigate the association of Arbuscular Mycorrhizal Fungi (AMF) and diversity in Rambutan Tangkue (Nephelium lappaceum L.) in Lebak Cilayang, Banten. This research was conducted from July 2018 to January 2019. The rambutan soil rhizosphere and roots were sampled from two stations and the AMF spores were trapping and extracted by Wet sieves and decantation techniques The results showed that the spore density Spore density varies between 2012-372/100g of soil. About four genera has been successfully isolated: Glomus, Acaulospora, Gigaspora and Scutellospora. Glomus is the most dominant genus in the Rambutan Tangkue rhizosphere.