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The use of cobalt ferrite as a filler in ferrofluids was proposed as a smart material with magnetic review for antimicrobial applications. Referring to X-ray diffraction data, the nano particle of Co0.4Fe2.6O4 shows an entirely magnetite phase with a particle size of about 9 nm. Filler morphology shows agglomeration with an average particle size distribution of around 41 nm. Claims for filler elements are made through element mapping. The magnetic properties of filler and ferrofluids are examined through hysteresis curves, resulting in both superparamagnetic conditions. Antimicrobial activity of ferrofluidics is carried out through the diffusion method on three types of bacteria and one type of fungus with a view of the diameter of the inhibited zone observed. Based on this test, a relatively high inhibitory zone value was obtained and could be recommended as an antimicrobial material.