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Abstract Liver cancer is the second most common cause of cancer deaths worldwide. Treatment that has been carried out to date was chemotherapy with cytostatic drugs, radiotherapy and surgery which still have many disadvantages. Bandotan Herbs (Ageratum conyzoides L.) containing flavonoid compounds that can be used as chemopreventive agents. Objective: This study was conducted to explore the chemopreventive activity of BHCF in DMBA-induced mouse rat. Methods: The simplisia macerated using ethanol 70% followed by fractionations using chloroform. The phytochemicals identification of BHCF is done by the TLC method. Molecular docking test of nobiletin compounds against VEGF proteins, COX-2 and C-Myc compared to 5Fluorouracil. The carcinogenesis test required 20 mice tails to be divided into 5 feeding groups. Induction is conducted on a per-oral using CMC-Na 0.5% 1 ml/200 gram, DMBA 20 mg/kgBW and BHCF at a dose of 750 mg/kgBW and 1500 mg/kgBW. DMBA is injected 2 times a week for 5 weeks. Histological observation is done by the Immunohistochemistry and Haematoxylin-Eosin methods. Results: The TLC results state that BHCF contains flavonoid as a secondary metabolites. Molecular docking proves that nobiletin is better in inhibiting the expression of VEGF with an affinity value -7.6 kcal/mol. Induction of DMBA causes moderate over-expression of VEGF against liver tissue. Histologically, the introduction of BHCF at a dose of 1500 mg/kgBW provides better improvement degrees of histology than 750 mg/kgBW. Conclusion: The presence of flavonoid can be used as chemopreventive agents for liver cancer based on in silico and in vivo assay.