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This study aimed to determine the growth and production of Doulu Cultivar Garlic of MV2 generation. This research was conducted in March-July 2019. The seeds used in this study came from MV1 Garlic which given Gamma Rays at dose of 6 Gy (G3), 8 Gy (G4) dan 10 Gy (G5). Planting seeds in carried out in a research center for vegetables crops, Tongkoh, Berastagi, North Sumatera. The design used in this research is Non Factorial Randomized Block Design with 4 replications. This research uses 4 levels of treatment in accordance with the source of the seeds the dose 0 Gy (G0), 6 Gy (G3), 8 Gy (G4) dan 10 Gy (G5). The seeds came from 67 tubers consisting of 340 cloves. The parameters observed are age of growth, height of plant, number of leaves, number of stomata, diameter of stem, weight of cloves, diameter of cloves, and number of cloves. Data from this study were analysed with Anova and Least Significant Different test (LSD test). The results showed that the growth and production of plants significant of effect based on seed by radiation doses that are on height of plants, number of leaves, diameter of stem, weight of bulbs, diameter of bulbs, weight of cloves, diameter of cloves, structure of bulbs, and shape of bulbs of cultivar Doulu garlic but no significant on age of growth, number of stomata, and number of cloves.