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This study aims to explore, study and identify information about character education of environmental care through biology learning. This research was conducted on biology learning, the sub-chapter of ecosystem in class X (Ten) at SMK Negeri 1 Pusakanagara, Subang. This study uses a qualitative approach with a case study method. Data collection techniques are carried out by observation, interviews and document analysis. Data are analyzed by stages: data collection, data reduction, data presentation and conclusion drawing; then the validity was tested using triangulation. Based on the results of the study, it was found that: 1) In biology learning planning contained values of environmental care on learning goals, learning processes and learning evaluation, 2) During the learning process the teacher taught and instilled character values of environmental care through information transfer (value), habituation, modelling and conditioning, 3) In evaluating learning, the character values of the environmental care are cultivated through cognitive, affective and psychomotor assessment. This paper is expected to stimulate research and develop education science and practice in Indonesia.