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This aim of this study was to apply water balance formula for calculating plant water requirements and the plant coefficient (Kc) of Annona muricata L. The Annona muricata L. was farmed at Nawungan orchard in Selopamioro village Imogiri sub-district. Village monographs, 1: 25,000 scale Indonesian Earth map and climate data were used to gain the purpose of study. An Automatic Weather Station (AWS) was installed in Nawungan orchard to record rainfall, air relative humidity, wind velocity, solar radiation, as well as soil moisture every fifteen minutes. A set of surface runoff equipment was set in Annona muricata L farming land. Infiltration test was conducted in nine selected location of Annona muricata L farming land and later it used to find soil percolation value. The purposive sampling method was adopted to assign the nine locations of infiltration test. The reference evapotranspiration (ET0) was calculated by Penman-Monteith method. Later, water balance formula was applied to determine the actual plant water requirements (ETa) and plant coefficient value (Kc). This study proved reference evapotranspiration (ET0) the study area was 5.74 mm/day, the actual crop water requirement (ETa) of Annona muricata L was 7.98 mm/day. Kc of Annona muricata L was 0,557 mm/day.