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Granular Activated Sludge - Sequencing Batch Reactor (GAS- SBR) has the ability to treat wastewater with high organic pollutant. The aims of this study to determine the effect of aeration time to remove parameters of COD, BOD, TSS and Total Ammonia in chicken slaughterhouse wastewater using GAS-SBR. The artificial wastewater treatment used as represent of chicken slaughterhouse wastewater in this processing is artificial wastewater made from a mixture of sucrose, KNO3 and KH2PO4. GAS formation was form by reacting activated sludge with slow stirring at 20 rpm. SBR has a total work volume of 45L which is operated in 5 stages (fill – 0.25 hours, aeration time varied for 2 hours, 4 hours and 6 hours, settle - 0.25 hours, draw - 0.25 hours and idle - 6 hours). The results showed that the variation of aeration time is optimum at 2 hours with the efficiency of COD removal was 72.83% (influent of 6016 mg/L), BOD was 72.23% (influent of 3614 mg/L) and TSS was 59.8% (influent of 2.8 mg/L). For total ammonia removal, the variation of aeration time is optimum at 4 hours resulted with efficiency of 65.85% (influent of 2.29 mg/L)