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This study intended to develop the entrepreneurial characters of UNNES Chemistry students through the implementation of Natural Product learning integrated with Ethnoscience and STEM (Ethno-STEM). This research is a descriptive-qualitative study subjected to Chemistry education students who joined the Natural Products and Chemical Entrepreneurship course. The research subjects were introduced to the project-based learning integrated with Ethno-STEM concerning concepts related to terpenes, steroids, flavonoids, and alkaloids; also, the introduction of natural chemical isolation and distillation processes both in the community and laboratory. The students were assigned a project of chemical batik production, i.e., batik with chemical structure patterns followed by evaluation and assessment. There were six best batik designs proposed to one of the traditional batik centres named “Zie” to be printed by using batik printing machine. These designs are said to be worthy of production; in other words, they are valued economically. The research results indicated that the Natural Product learning integrated with Ethno-STEM was able to well develop the students- mastery concept of chemistry, creative and innovative thinking, perseverance, and conservation of national culture