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Bone is a by-product that causes bone heaps. Chicken bone contains 24-30% of Ca and 12-15% of P, so it can be used as a source of P and Ca, but not yet available. To dissolve it needs the addition of organic matter. Latosol is a soil that lacks of P and Ca. This study aims to determine the effect of application of flour from chicken bone waste and organic matters on the availability of P and Ca of Latosol and growth of corn. The study was carried out in the greenhouse of UPN “Veteran” Yogyakarta Faculty of Agriculture, on January to April 2019. The study used a completely randomized design of two factors. The first factor is the dose of chicken bone flour, are without chicken bone flour (A0), chicken bone flour 2% (A1), 4% (A2), and 6% (A3). The second factors is organic matters, are without organic matter (B0), compost 3% by weight of the soil (B1), and manure 3% by weight of the soil (B2). Each treatment was replicated 3 times. Application of chicken bone flour had a significant effect in increasing pH H2O, C-Organic, available-P, and available-Ca, plant height and plant dry weight. Application of organic matter had a significant effect in increasing pH H2O, C-Organic, available-P, available-Ca, plant height and plant dry weight. The combination of chicken bone flour and organic matters interacted each other in increasing available-Ca, while there are no interaction to affect pH H2O, C-Organic, available-P, plant height and plant dry weight.