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Dengue fever is a dangerous disease, can cause death in a relatively short time. The disease is caused by dengue virus that enters the human body through the bite of Aedes sp. Gondokusuman sub-district is one of dengue endemic areas in Yogyakarta City. The purpose of this study to determine the environmental health conditions of Dengue Fever patients, efforts made, and the spread of the location of the incidence of patients in 2014, 2015 and 2016 with Geographic Information Systems (GIS). This research is an observational research which the result is analyzed descriptively and analytically. The subjects of the study were 172 patient homes, the samples were taken in total sampling. The data were collected and analyzed by SPSS using Pearson correlation test with 95% confidence level. Taking sample point using GPS then plotted and analyzed visually. Descriptively, there are 15 houses with larva, the effort made for the prevention of the existence of larvae divided into 3, good with 18 houses, enough with 23 houses and less with 131 houses. The form of the house is divided into 3 categories namely modern homes, houses and slum houses. Based on Pearson Correlation Test, with significance level 0,05, it was found that there was correlation between house condition of child-teenager and adult with prevention effort of larva in Gondokusuman sub-district, and there was no significant correlation between house condition of man and woman with efforts to prevent the presence of larva in Gondokusuman sub-district. Based on the analysis of the distribution of the location of Dengue Fever patients with Geographic Information System (GIS), the pattern of Dengue Fever spread is very high in Klitren. Based on the zoning level of prevention efforts and incidents that successively for 3 years concluded that Kelurahan Terban entered in Zone I is located on the edge of the Code river, zone there are RW areas that every year there are patients with Dengue Fever, less prevention efforts, and it is a densely populated settlement.