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This study investigated the response of Hibiscus Leaf Meal (HLM) and Direct-Fed Microbials (DFM) supplementation on nitrogen metabolism and rumen microbial synthesis by local sheep. Thirty six male local sheeps aged ±2 years (28,01±2,61 kg) were fed concentrate (3% of body weight) supplemented with HML twice a day, amoniated rice-straw supplemented with DFM were given ad-libitum were assigned randomly to nine treatment in an experiment of 3×3 factorial design. The first factor was the use of DFM (P0=control, P1=DFM AMS, P2=DFM RJ) and the second factor was HML level (W0=0%, W1=0,24%, W2=0,24% of concentrate DM). Measured variable were nitrogen digestibility (ND), nitrogen retention (NR), rumen microbial synthesis (RMS), and efficiency of rumen microbial synthesis (ERMS). There were significant interaction (P<0,01) between DFM and HML suplementation on both RMS and ERMS. While, there were no interaction on ND and NR. The highest RMS and ERMS were achieved on P0W1, there were 6,650±1,18 gN/day and 16,44±5,03 gN/kg DOMR, respectively. Although there were no interaction (P>0,05) between DFM and HLM on ND and NR, DFM supplementation significantly (P<0,01) affected both ND and NR. The most efficient of them were achieved on P0 treatment, 55,13±11,86% (W2) and 9,12±1,76 gN/day (W1), respectively. Based on these results, it could be concluded that determination of HLM level for RMS enhancement depend on DFM usage. At the HLM level 0.24% of concentrate DM, it was advised to use DFM RJ. In contrast, at HLM level 0,48% or higher, it was advised to use DFM AMS. Further experiment to determine maximum level of HLM on DFM AMS use is needed.