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Estuary is a coastal waters where the river mouth meets the sea and where fresh water mixes with salt water from the sea. Estuary forms a transition zone between the river environment and the marine environment. The mix of seawater with fresh water makes the estuary region unique, with the formation of brackish water with fluctuating salinity. The purpose of this study was to determine the distribution of salinity and temperature as well as the relationship of the effect of temperature on salinity in the Muara River area during the highs and lows conditions. Data retrieval is done in the Jeneberang River Estuary area with a distance between 200m points using the ADCP (Acoustic Doppler Current Profiler) and CTD (Conductivity Temperature Depth). The pattern of spreading salinity and temperature is described using the ArcGis 10.3 program. The results showed an increase in salinity found in the Jeneberang estuary area during tidal conditions and receding conditions, as well as the closeness of the relationship between temperature and salinity which showed a directly proportional result. Based on water clarification, the Mueberang River estuary area can be categorized as brackish water.