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The purpose of this study was to find out the concept of sustainability in the frame work of pedagogical content knowledge (PCK) from prospective biology teacher in teaching the respiratory system. Data is collected qualitatively through documents of learning tools and observation video during the learning process. This study uses two prospective female biology teachers who were in their final year at the undergraduate level of Biology Education. The results reveal that candidates have the same knowledge about sustainable concepts, but their representation of understanding the concept of sustainability in PCK frame work is very different. This can be seen in terms of context, learning strategies, learning models, evaluations and out comes.