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The study aims to (1) to find out the stages of developing biology learning tools based on character education integrated with sustainable local wisdom, (2) to determine the validity, practicality and effectiveness of biology learning tools based on character education integrated with sustainable local wisdom, and (3) to find out the effect of biology learning tools based on character education integrated with sustainable local wisdom. The trial of this research product was conducted to 11th grade students of SMAN 2 Kota Bima in the school year 2018/2019. This research is a developing research which is based on Thiagarajan development model which consists of 4 stages are define, design, develop and disseminate. The instruments of this research are validation sheets, observation sheets, attitude assessment instruments, cognitive tests, and student response questionnaires. The results of this study indicate that developing biology learning tools based on character education integrated with sustainable local wisdom is valid, practical and effective to enhance 21st century attitude of students.