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Effect of Centella asiatica to the Glucose Transporter 4 and Osteocalcin on the Rotenone-Induced Zebrafish Larvae (Danio rerio) Stunting Model
Vanda Primaditya1,2, Dyah Woro Kartiko Kusumo Wardani 1,3, Dianita Primihastuti 1,4, Fitra Arsy Nur Cory-ah 1,5, Linda Ika Puspita Ariati 1,6, Yuningsih1,7 and Zakiah1,8, Husnul Khotimah 9,a), Mohammad Muljohadi Ali9,Wibi Riawan 10

1Master Program of Midwifery, Faculty of Medicine Universitas Brawijaya, Indonesia 2Midwifery Academy of Berlian Nusantara, Magetan, Indonesia
3 Institute of Health Science Sint Carolus, Jakarta, Indonesia
4Institute of Health Science William Booth, Surabaya, Indonesia
5Midwifery Academy Poltekkes Kemenkes Mataram, Mataram, Indonesia
6Midwifery Academy of Jember, Jember, Indonesia
7Midwifery Academy of Dr. Soebandi, Jember, Indonesia
8University of Darul Ulum Jombang High Islamic Boarding School, Jombang, Indonesia 9Laboratory of Pharmacology, Faculty of Medicine, Universitas Brawijaya, Indonesia 10Laboratory of Biochemistry-Biomolecular Faculty of Medicine, Universitas Brawijaya, Indonesia


Abstract

Stunting is the failure of linear growth during the first two years life. Rotenon is one of the pesticide causes stunting. Rotenon as the Endocrine Disrupting Chemicals (EDCs) and 1 complex mitocondria inhibitor, causes the decrease of ATP, increase the ROS and caused oxidative stress. Centella asiatica is a plant contains high antioxidant and cause the balance oxidant in cell so oxidative stress can be decreased. The purpose of this study is to know the pegagan etanol effect toward the rotenon induced zebrafish larvae stunting model toward expression increasing of Glucose Transporter 4 (GLUT 4) and Osteocalcin. The research proved rotenon [12,5 ppb] can cause stunting of zebrafish larvae which included age 6 and stay until 9 dpf. The expression of GLUT 4 and Osteocalcin in zebrafish larvae which rotenon-induced decrease significantly compared with control group. The expression of GLUT 4 increase significantly by adding pegagan concentration 5μg/ml, while the expression of osteocalcin increase significantly by adding pegagan concentration 2,5μg/ml and 5μg/ml. Rotenon [12,5 ppb] can induce stunting of zebrafish larvae (Danio rerio). Addition of pegagan etanol extract can give effect for stunting zebrafish larvae through increased expression of GLUT 4 and Osteocalcin. This is indicated by an increased in body length.

Keywords: Centella asiatica, rotenon, zebrafish, stunting, GLUT 4, osteocalcin

Topic: Biomedical Science

Link: https://ifory.id/abstract/hDGycCm9tuk6

Conference: International Conference on Life Sciences and Technology (ICoLiST 2019)

Plain Format | Corresponding Author (Husnul Khotimah)

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