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Dental caries is one of the susceptible problem among preschool children. Some parents feed formula and add sugar, they gave the formula while the children lay down even until they fall asleep, these caused dental plaque. Plaque on the teeth that will trigger the appereance of dental caries. The aimed of this study was to analyze the relationship between parental behaviour in formula feeding with bottle towards dental caries among preschool children in Infant Integrated Health Services Sumerta Kelod, Public Health Centre I, East Denpasar. Correlative analytic design was used in this study with cross sectional approach. Respondents were 78 parents and their preschool children selected using consecutive sampling technique. The instrument used in this study was a self-developed questionnaire and observation form. The respondent answered by their self-completed questionnaire and all respondents completed questionnaire. The observation by researcher. The result showed that among 78 respondents, 37,2% were housewife, 47,4% were 30 – 39 years old (ranged from above 20 years old), 48,7 % of parents are in moderate categorical, 47,4 % of preschool children had dental caries and 57,7% of preschool children had dental plaque. There is a correlation between parental behaviour and dental caries with p value < 0,05 (P=0,000) and correlation 0,605. Decrease in parental behaviour, increase dental caries in preschool children. It is expected that health practitioners provide further information about dental care maintain both of parents and their children.