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Azadirachta indica, A., Juss is a medicinal plant that is used traditionally for some disease, especially its leaves are used to treat a rheumatic diseases and lower blood uric acid levels. This study has been carried out to examine antihyperuricemia activity of ethanol extract, water fraction, ethyl acetat fraction and n-hexane fraction of the A. Indica leaves in male mice of Swiss-Webster strain. Extract and fraction doses used were 125, 250, and 500 mg/kg bw. The tests were conducted on mice suffering from hyperuricemia induced by potassium oxonat intraperitoneally and chicken liver juice orally. Measurement of blood uric acid levels were performed every hour for 4 hours after being given test preparations. The results showed that the ethanol extract and the fractions lowered blood uric acid levels. The n-hexane fractions at the three doses showed the highest activity. Then, the most active fraction, n-hexane fraction, was tested for its inhibitory activity on xanthine oxidase enzyme to determine its mechanism of action. The results showed that the n-hexane fraction inhibited uric acid biosynthesis by inhibiting the activity of xanthine oxidase enzyme with the IC50 value of 132 microgram/mL.