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Thailand and Ungu cultivars are two of the many cultivated papaya cultivars in East Java. In addition to fruit, papaya leaves are also widely used as a traditional insecticides. The purpose of the study was to determine the active compounds, total phenols, total flavonoid, and antifeedant activity of papaya leaf extract of those cultivars against Spodoptera litura F. larvae. Papaya leaves were macerated with methanol. Phytochemical of leaf extract determined using LCMS. Total phenol and total flavonoids were measured based on the spectrophotometric method. Antifeedant activity was tested on instar 3 phase larvae with choice method. Feed leaves sprayed with different concentration of papaya leaves extract and repeated 10 times. The screening result showed that there are 62 compounds detected in Ungu extract and 60 compounds in Thailand extract. The highest total phenol content was found in Ungu extract. Meanwhile the highest total flavonoid content was detected in Thailand extract. The treatment of 40% and 50% of both extracts significantly inhibited eating activity to 100%. Statistically, the combination between the type of extract and concentration showed significant antifeedant activity. According to the result, the different of antifeedant activity of exctract caused by the difference of the phytochemicals content . The extracts of Thailand and Ungu papaya leaf had the potential to be antifeedant for S. litura larvae.