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The purpose of this study is to conduct a quality control analysis of carded and combed yarn produced using the six sigma method. The approach used in working with the Six Sigma method is DMAIC (define, measure, analyze, improve and control). The results obtained from the carded and combed yarn products are there are 4 types of defects, such as the strength of the thread does not match the standard, contami, bad rolls and tangled thread. The average DPMO value is both threads of 7,786 with an average sigma level of 4. The root causes of product defects are operators fatigue, age of machines, no machine checks, cotton fibers from the eastern countries and lack of ventilation. The conclusion obtained from 4 types of strength defects is that the strength defects do not match the standard is the most dominant type of defect. In addition, the results of the DPMO value and sigma level indicate that the product quality is good, exceeding the average sigma level of the company in Indonesia.