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Indigofera tinctoria is a source of natural blue indigo color that belongs to Fabaceae family. Natural dyes extracted from plant leaves containing indican compounds. The growth and yield of this plant are determined by the availability of nutrients. The way to improve growth and yield of indigofera is using rhizobium and mycorrhiza. This study evaluated the Indigofera tinctoria growth as the response of mycorrhizae (AM) and rhizobium, nodulation and productivity. Indigofera tinctoria plants were cultivated in the Faculty of Agriculture Greenhouse with a complete randomized block design with two factors. The first factor was the inoculation of Rhizobium sp with 3 levels (0.1 and 2 g per plant). The second factor was mycorrhizal inoculation with 3 levels (0, 5 and 10 g per plant). The results indicated that rhizobium significantly affected plant growth. Rhizobium increased plant height, number of leaves and number of shoots from 2 week after planting (WAP) to 12 WAP. Inoculation of Indigofera tinctoria with AM fungus resulted a significant increase in nodulation. Root growth was longer with mycorrhizal inoculation. In addition, mycorrhizae also enhanced canopy and root biomass at 6 WAP. Mycorrhizal and rhizobium inoculation of indigofera tinctoria plants significantly increased plant growth, number of nodules and biomass. Inoculation doses in this study were linearly correlated with growth, nodulation and results. Thus, the combined inoculation with mycorrhiza and rhizobium showed a synergistic effect. This combination could be applied to improve plant growth.