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Succinic acid is one of the 12 top chemicals building block by the United States Department of Energy that has applications in various industries, such as food, pharmaceuticals, petrochemicals, cosmetics, and agriculture. One of the bacteria that can produce succinic acid is Actinobacillus succinogenes, which is included in the Actinobacteria phylum (0.86%) in ruminants, especially in the cattle rumen. Actinobacillus succinogenes has the ability to produce natural succinic acid with high yield value, productivity, and fermentation efficiency with renewable resources. Succinic acid can be produced through fermentation configuration from bacteria isolate with the help of OPEFB biomass as the carbon source. The fermentation process in this study uses a Semi-Simultaneous Saccharification and Fermentation configuration which is a combination of Separate Hydrolysis and Fermentation and Simultaneous Saccharification and Fermentation configuration to increase the yield of succinic acid. This study aims to obtain bio-succinic acid using bacteria isolates from the goats rumen through SSSF configuration and obtain the initial glucose concentration, nitrogen source concentration, and concentration of pH regulating compounds to optimize the concentration, yield, and productivity of succinic bio-acid. This research will begin with the isolation of bacteria from the goat rumen, OPEFB preparations, and fermentation process will be carried out.