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Abaca banana is one economic fiber plants,the lack of abaca banana supply has encourage the expansion of abaca banana plantation. To support the availability of plants material,propagation by tissue culture technique being a good alternative for mass production. This reserch was aimed at studying the responses of shoot production of abaca banana as result of effect of type of various charchoal and sukrose at MS medium through in vitro method. The study was conducted in the Laboratory Tissue Culture Department of Agrotechnology UPN”Veteran”Yogyakarta .Results showed that with addition of pro analys active charchoal 2 /L and saccharose 30 g/L can significanly increase number of leaves, the lenght of planlet, the number of shoot and the length of shoot.