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Unstable emotions often occur during the pregnancy and place the pregnant women in a stress transition and tends to a vulnerable condition, which can increase proinflammatory cytokines so as to trigger preterm labor. The pregnancy period is a condition to get closer to God and make life more meaningful by using religious beliefs as a strong coping mechanism. This study aims to prove routine midwifery care plus psychoeducation Żikir further reduces perceived stress and the level of IL-6 Primigravida women compared to routine midwifery care. This study was an experimental study. A number of 37 participants as intervention group and a number of 38 participants as control group. The perceived stress obtained using Perceived Stress Scale and IL-6 levels measured using ELIZA kits in the third trimester of pregnancy. Statistical test using independent t test to compare delta score. The result showed mean difference between groups on the end of interventions, perceived stress 4.520 CI 95% (-7.687 – [-1.353]) and p value is 0.006. The difference of the IL-6 between groups 8.371 CI 95% (-11.361 – [-5.38]) and p value is less than 0.001. Conclusions: Routine midwifery care plus psychoeducation żikir has more decrease perceived stress and IL-6 levels in primigravida women