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Environmental pollution caused by plastic packaging materials has been alarming, causing the researchers to make films that are easy to decompose. Edible film is a thin layer that serves as a protector of food and barriers to the transfer of mass, such as water vapor, and oxygen. Taro tuber is one of the raw materials in the manufacture of edible film which has great potential because it has high starch content. Most edible films made from starch have deficiencies that are fragile so plasticizers need to be added. Addition of glycerol is more efficient in improving physical and mechanical properties such as tensile strength and elongation because it can make edible film more flexible and strong against physical damage and can produce edible which has a high transparency. This study aims to determine the effect of concentrations of taro starch and glycerol on the physico-mechanical properties of the edible film produced. The study was conducted using factorial randomized block design with two factors. The first factor was the concentration of taro starch (2, 2.5, and 3%) and the second factor was the concentration of glycerol (25, 30, and 35%). The parameters tested were thickness, tensile strength, elasticity, solubility, transparency, and water vapor transmission rate (WVTR). The results of this study indicate that there is an interaction between the concentration of taro starch and glycerol to the parameters of tensile strength, elasticity, solubility, WVTR and transparency. The best results were produced by treatment of 3% starch and 25% glycerol with a character thickness of 0.15 mm, tensile strength of 10.63 MPa, elasticity of 60.21%, solubility of 23.48%, WVTR of 25.37 g/m2/24 hours and transparency 2.23 mm-1.