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OBJECTIVE Dura mater is a special tissue that has a critical function in brain anatomy and physiology. This tissue contained some numerous cells, stem cells, and growth factors. This research investigate the protein interaction that contribute to dura mater healing process. METHOD To identify and predict in silico of functional protein interaction in dura mater healing process, we use available analysis software to perform the protein-protein interaction (PPI) analysis (http://gpsprot.org/index.php). GPS Protein is an interactive platform for visualizing human protein interaction by integrating HIPPIE and CORUM databases. We excluded HIV-1 proteomic and RNAi databases, hence focused on human PPI (Confidence level 0.75). Two proteins were inputted as query to identify the potential protein network in dural healing according to previous studies, i.e fibroblast growth factor-2 (FGF2) and transforming growth factor beta-1 (TGFB1). RESULTS In previous study used dura mater cells culture found the role fibroblast in dura mater healing process, FGF-2 and TGFB as the promoters of dural closure. PPI results showed the more important role of TGFB1 with more interactions of TGFB1 to some remodeling proteins (COL, ITG, MMP etc). TGFB1 encoded regulates cell proliferation, differentiation, growth, modulate expression and activation of other growth factors, also induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types. CONCLUSION This bioinformatics approach is an efficient and cheaper method for analyzed the molecular aspect of protein that have a special contribution in dura mater healing process. This results could help to focused the further research in more complex laboratory examinations.