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Anaerobic digestion is a biogas production process that can be used as a sustainable alternative energy. Anaerobic digestion utilized vary type of organic waste as substrate for reaction process. Conversion process of organic wastes into the energy could solved the environmental problems. Food waste is one of the high organic content waste which become global environmental issue. In this research, dry anaerobic digestion system used to determined biogas production potential from food waste with cow manure as the inoculum. Three variation total solid (TS) of substrate and inoculum were used to compare the biogas production performance. After 24 days incubation time on 25°C, result of biogas production from 1:1, 2:1, and 1:2 ratio of food waste and cow manure were 144 mL/gr TS, 73.46 mL/gr TS, and 110.27 mL/gr TS respectively. Based on those result, food waste and cow manure ratio 1:1 TS suggest to be used on improved dry anaerobic digestion system.