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This study aims to describe the results of design optimization and characterization of mechanical properties of knee implant with SPSS software and characterization using SEM, CRD, and XRF. Optimization is focused on optimizing the total deformation value from human activity of walking, jumping, and downstairs to determine the optimum design of the knee implant. While the characterization of the material is intended to determine the morphology using SEM, chemical composition using XRD, and crystal structure using XRF. The finding of this study is that optimization of total deformation shows that the first design has the lowest maximum average deformation value, that is 0,00014836 mm. While the results of SEM characterization showed that there were many parallel strokes on Ti6Al4V alloys, so that they were not resistant to damage from abrasive particles. The XRD test shows that the crystallinity peak is at position 40.5189182 degree which is indicated by the crystal orientation index 200 reaching 29.35 counts (cts), and Full Width Half Maximum (FWHM) at an angle of 0.2880282 degree which has an atomic distance along the length of 2.22455 amstrong with a relative intensity of 100%. And the XRF test shows the highest chemical content of Ti6Al4V alloy is Ti, amounting to 85.12%. The limitation of this study is that optimization is based on total deformation on three human activities. While the characterization is done by using SEM, XRD, and XRF tests. Optimization and characterization of implants can be the basis of the process of manufacturing knee implants. The results showed that the first implant design was the optimum design based on the total deformation. Then in terms of characterization, it appears that the material is in accordance with the human body.