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Heat loss occurs due to the temperature difference between the fluid flowing in the pipe and the temperature outside the pipe, it can also occur due to inappropriate material selection and insulation thickness. Insulation is a method for reducing the rate of heat transfer from inside a pipe to the outside of the pipe. Valve is an object that is often encountered in un-insulated conditions so that there is a lot of heat loss in this section. Therefore, an insulation method and the right insulation material are needed to prevent heat loss. The study was conducted to see the effect of two glass wool and rock wool insulation materials on heat loss. The study used a simulation method using NAIMA 3E Plus software. The output of the simulation is the value of heat loss on glass wool and rock wool material. Data analysis using two-way ANOVA method assisted by Minitab software. The results show that the higher the temperature, the greater the heat loss value and the thicker the insulation layer, the smaller the heat loss value. Insulation material is better to use rock wool.