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Antibiotics are the main substances that used to treat infectious diseases such as typhoid fever. Evaluation of antibiotic use is needed to reduce the occurrence of resistance. Antibiotic resistance can be fatal. Failure respond to treatment can lead to prolonged illness, increased risk of death and extend the length of stay. Besides having an impact on morbidity and mortality, antibiotic resistance also made economic and social impact. This study is to examine the relationship between the accuracy of the duration of antibiotic administration in typhoid fever and the length of stay. Method: A Retrospective non-experimental study with cross-sectional design. Medical records were assessed the accuracy of antibiotic duration by Gyssens criteria. From 75 medical records that fulfill the inclusion criteria, 64 patients had the right duration of antibiotics. Length of stay varies from 2 days to 7 days. The most widely used single antibiotic was levofloxacin 27 (36%). The results of the chi square analysis obtained a value of p> 0.05. The conclusion: there is no relationship between the accuracy of the length of antibiotic administration and the length of hospital stay.