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The aim of the research is to investigate the effect of four different energy level in the ration into the calpain activity of Jawarandu does. The research was done during 5 months in the Experimental Farm of the Faculty of Animal Science, Universitas Jenderal Soedirman. The research material used was 16 heads of the Jawarandu doe with the aged 2.5−3 years. All the animals were randomly assigned to the ration treatment which forms four the different energy levels (82.26% TDN, 85, 87.93, dan 90.74% TDN). The replication of each treatment was four times. Variable measured was a calpain activity on the muscle of Longissimus dorsi. General linear model (GLM) of the SPSS was used to analysis variable measured. Energy content 1.63McalME/heads/day and 1.92McalME/heads/day as well as 1.73McalME/heads /day and 2.06McalME/heads/day were increased of the μ-calpain and m-calpain activities at the Longissimus dorsi muscle, respectively. However, there was decreased of the calpastatin activity at the Longissimus dorsi muscle. Different energy content of the ration increased the μ-calpain and m-calpain activities at the Longissimus dorsi muscle and of those decreased calpastatin activity.