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Atherosclerosis is an inflammatory of artery wall by lipoprotein oxidation. Food with high content of fat is one of causes increases the level of LDL. The increase in LDL level increases free radicals in form of ROS and causing to be easily oxidized into Ox-LDL. Ox-LDL triggers the activation of macrophage and Th17 cells to secrete pro-inflammatory cytokine IL-6. Treatment for atherosclerosis uses simvastatin could influence the activity of nervous system, so that safe treatment with herbal treatment, one of examples is single bulb garlic. The research aims to find out the influence of single bulb garlic oil on the decrease ROS thus it could inhibit IL-6 secretion and the number of lymphocytes in mice with high fat diet (HFD). Mice were feed with high fat diet for 45 days and treated for 30 days. Mice were grouped into 6 groups, normal, HFD with simvastatin 26 mg/kg BW, single bulb garlic oil 0 mg/kg BW, 12.5 mg/kg BW, 25 mg/kg BW, and 50 mg/kg BW. In the end of treatment, mice were dissected for collection of (1) serum in order to measure its level using ELISA with ROS and IL-6 antibodies, (2) aorta from heart to measure its expression using IHC-F with IL-6 antibody, lymphocyte from bone marrow and spleen was calculated for the number of cells using light microscope. The result indicated that the single bulb garlic oil has influence on decrease of ROS level, IL-6 level, IL-6 expression and the number of lymphocyte.