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The consumption of beef in Indonesia continues to increase every year, but national beef production is only able to meet 65% of domestic demand. Therefore, a new innovation is needed to increase the productivity of cattle, one of them by twinning births in cattle. The identification begins with taking blood samples of cattle. Then isolation of bovine blood DNA, amplification of sequence ovulation rate gene, horizontal electrophoresis, and then sequencing. The sequencing results were then analyzed using bioinformatics software to see the sequence variation and thus found predictions of established polypeptides which might play a role in the rate of ovulation of Limousin cattle. Based on the results of the analysis, there was a sequence variation and the prediction of the polypeptide formed was Cytokine (TNF Tumor Necrosis Factor). This shows that the variations that occur cannot change the polypeptide formed, because the polypeptide formed from the three samples is a class of cytokines that have a function for ovulation in mammals. Thus it can be concluded that the variation of gene sequences does not change gene function can be said to be gene conserve and conservation and breeding to increase cow productivity.