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The aim of the study was to produce Socio-Scientific Issues (SSI) based teaching materials on biotechnology material that could improve students scientific literacy. This research is an R & D study with ADDIE design (Analysis-Design-Develop-Implement-Evaluate) using one group pretest-posstest design in a limited implementation. The study was conducted at MTsN 5 Cianjur in class IX students. Data was collected using validation sheets, observation sheets, questionnaires, and scientific literacy tests. Data analysis was conducted in quantitative descriptive. The results of expert validation by showing SSI-based science teaching materials have valid criteria and are very suitable for use on material aspects (CVI = 0.94) and graphics (CVI = 0.98). The average percentage of test scores for all aspects of the overall test is 55.89%, so the instructional materials developed are included in the criteria for instructional level instructional materials. The product effectiveness test results obtained by N-Gain scientific literacy of 0.60. Therefore, it can be concluded that SSI teaching materials are effective enough to improve scientific literacy in students. The product practicality test results are based on the teachers and student response are 85.31% and 85.67%. This results indicates that teaching materials are practically used in learning.