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Shallot plants (Allium ascolonicum L) are included in plants that are propagated vegetatively and Allium sp family. For seed production and elimination of viral systemic disease it has been carried out in vitro / micropropagation. The research was conducted at the Vegetable Research Institute Tissue Culture Laboratory in August of December 2018. The aims of the study was to see the effect of phytohormon picloram and BAP on the growth of meristematic Variety Maja. The treatment were MS media (1962) + MS vitamin + sucrose 30 g / l + IAA 2 mg / l + kinetin 2 mg / l + GA3 0.01 mg / l + Myo inositol 100 mg / l + CaP 2 mg / l + gel rite 2 g / l, pH 5.7. The treatment of phytohormon picloram (0,1,2 mg / l), BAP (0,1,2 mg / l), number of composition of treatment media were 9. The results of visual observations (1) percentage of contamination up to 8 MST 30- 50 %, generally caused by fungi or bacteria, (2) visually the proliferation percentage between 65 - 100%, explant growth in M1 to M9 media one shoot per explant. And the DAS ELISA test plant detected were infected with OYDV, SYSV 36.36% d.d 53.85%.