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Cryopreservation of Bone Marrow Stromal Cells (BMSCs) is needed to maintain the biological properties and minimize isolation repeatedly. The aim of this study was to optimize cryoprotectant agents to maintain the proliferative capability of BMSCs. This study used three group of combinations; 1) 90% FBS + 10% DMEM; 2) 90% DMEM + 10% DMSO; 3) 90% FBS + 10% DMSO and Cell Banker 1 (CB1) as a positive control. The study showed cell morphology of spindle, round and flatten-shaped. The cell viability in the second combination was 69.73% and 79.74%, the third combination was 68.08% and 74.85% while CB1 was 81.5% and 85% stored at -80oC and LN2, respectively. Cell density post-thawed in the second combination was 26.5x104 cells/ml and 31.25x104 cells/ml, the third combination was 24x104 cells/ml and 32x104 cells/ml while CB1 33x104 cells/ml and 35.5x104 cells/ml at -80oC and LN2, respectively. Cell proliferation capability differed significantly was (p<0.05). Population doubling time increases as long as with increasing incubation time. The use of the second and third combinations has the ability to recover almost as close to CB1 in post-thawed of cell proliferation capability so that it can be an option for cryopreservation of cells.