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Hibiscus rosa-sinensis is a plant that has flowers with many variations in size, petal shape, petal colors, and chromosome number. Chromosome number and morphology information is the key to study its flower morphology and physiology. Chromosome Image Analyzing System IV (CHIAS IV) is software that used for mapping and identifying chromosome automatically. This report presents the results of chromosomes analysis using CHIAS IV from four H. rosa-sinensis flower variations (single small red, single small pale red, single big red, and double red). Chromosome number and condensation patterns of individual chromosomes analysis suggested that these flower variations of H. rosa-sinensis exhibited polyploidy, with the smallest number found in single small pale red and the biggest number in single big red flower. CHIAS IV software is an effective tool to analyze chromosome images, especially to predict chromosome number, chromosome type, relative length, and condensation patterns of individual chromosome.