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Efforts to increase rice productivity in Indonesia are still constrained by attacks of plant pest organisms which cause low crop productivity. Diseases that often attack rice plantations include blast and leaf blight disease. Blast is caused by a fungus Magnaporthe grisea Cav. While leaf blight is caused by Xanthomonas oryzae pv.oryzae (Xoo). There are isolates of bacterial that have ability to provide resistance to disease attacks on plants, one of which is Paenybacillus polymixa. Based on this study, the aim of this study was to determine the ability of P. polymixa isolates in controlling the attack of Xanthomonas and fungi Magnaporthe grisea in vitro. The research stage is a test of bacterial inhibition of Xoo and M. grisea. The inhibition zone index in the antagonist test for Xoo pathogens in MO Plus was higher than P. polymixa. MO Plus inhibition zone index 1.87 while P. polymixa is only 1.20 at 7 days after incubation. P. polymixa bacteria have the highest index in inhibiting P. oryzae, which is 57.02. While the MO Plus inhibition index is 21.12