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This study aimed to obtain the characteristic of nanoparticles concentrate protein of snakehead fish (Channa striata) by addition of tween 80. The research consisted of two phases. First phase was the manufacture of nano concentrate protein dispersion from snakehead fish and second phase was the drying process of the concentrate using freeze dryer and characterization of physic-chemical properties of nano concentrate protein snakehead fish. The results of particle size measurement after using the sonication method and homogenization with the addition of tween 80 ranged from 331.6 to 353.0 nm. Based on the results of the particle size analyzer (PSA) the best value in the treatment of adding of 2% tween 80 with a polydispersity index value of 0.470, particle size distribution based on volume obtained by 2 size categories namely 101.8 nm and 449.4 nm and the average of smallest particle size ranged from 223.6 to 363.2 nm based on the results of the analysis of electron microscope scanning (SEM). The morphology of concentrate protein of snakehead fish based on the results of SEM analysis before the addition of tween 80 has abstract molecule, compact molecular structure (the molecules stick together) and the size was still in micrometer scale. While the addition of tween 80 showed that the particle structure of granules was not compact so the possibility of being broken down was still very high and in nanometer scale particle size. The physical characteristics of nano concentrate protein of snakehead fish before adding tween 80 included density kamba (0.52 g/ml), emulsion capacity (14.72%), water absorption (2.49 ml/g) and oil absorption (2.16 ml/g). After adding tween 80 the physical characteristics included density kamba (0.46 g/ml), emulsion capacity (48.15%), water absorption (0.42 ml/g) and oil absorption (2.00 ml/g). The chemical characteristics of nano concentrate protein of snakehead fish before the addition of tween 80 included, water (6.98%), ash (6.03%), fat (2.27%) and protein (85.10%). After adding tween 80 the chemical characteristics were water content (5.13%), ash (4.08%), fat (6.48%) and protein (88.28%).