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This study aims to determine the detection of the Aero gene in the bacterium Aeromonas hydrophilla as a candidate for bioremediator in dealing with hospital wastewater. This type of research is qualitative descriptive. The sample of this study is the bacterium Aeromonas hydrophilla which has the ability to hydrolyze proteins which are candidates for bioremediator agents. Samples were obtained from the Microbiology Laboratory, State University of Malang. Research Detection of the Aero gene in the Aeromonas hydrophilla bacteria was carried out in three places, namely the Microbiology Laboratory of Universitas Brawijaya for the DNA isolation stage, LSIH UB for the PCR stage and the Molecular Laboratory of Maulana Malik Ibrahim for Electrophoresis. The samples were analyzed using Zymo Research Quick-DNATM Bacterial Miniprep Kit for DNA isolation, Specific Primer for the Aero gene namely Aero 1a (forward) and Primary Aero 1b (Reverse) for PCR and Hyperleader VI for markers in analyzing PCR results in the electrophoresis process. The results showed that the Aero gene was detected at 48oC with a DNA concentration of 2.55 µg / ml, DNA purity of 1.53 µg / ml and the length of migration of 500bp DNA bands.