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The way to detect the growth disorder for children is by regularly weighing children, especially for under-five children. However in Buleleng Regency, mothers participant who aware for weighing under-five children were still rare. This case caused by lack of knowledge of them. It was important to improve mothers knowledge by health education using animated video, but Community Health Centre in Sukasada Buleleng never provide that kind of education. The purpose of this study was to find out the effect of health education using animated video towards the knowledge of mothers with under-five children about the importance of weighing under-five children to the health integrated post. This study implemented pre-experiment with one group pre-test and post-test design and was carried out in the area Juuk Mas with the total population 48 mothers with under-five children. Sample was selected through total sampling. Data were collected by using a questionnaire and analyzed by using the Wilcoxon Rank Test. The result showed that the majority of mothers had sufficient knowledge before they were provided with health education (Md: 6, min: 5, max: 8) and had good knowledge after they were provided with health education (Md: 9, min: 7, max: 10). The statistic result showed that health education using animated video had an effect in improving the mothers knowledge (ρ value <0,001).The conclusion of this research was health education using animated video can improve the knowledge of mothers with under-five children about the importance in weighing under-five children in the health integrated post. Therefore, in order to be effective, health educations are expected to be carried out by using animated videos. Mothers with under-five children are also expected to take their children regularly for weighing in the health integrated post.