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Tebing Siring SDN 3 students have a low ability to run, so the researchers concluded that Tebing Siring 3 SDN students have yet to have a good running speed. This study aims to determine the effect of interval training on the running speed of 40 meters in Tebing Siring 3 Elementary School students in Tanah Laut Regency. The research method used is the pre-experiment design method, with data collection techniques carried out through the initial test, giving treatment as many as 16 meetings and the final test. The population in this study were all students of SDN Tebing Siring 3, Tanah Laut Regency, class IV and V, amounting to 42 people, while the sample in this study were 20 people with a sampling technique using purposive sampling technique. The results showed that the pretest data running speed of 40 meters obtained an average = 7.1195 seconds with a standard deviation = 0.32830. In the posttest data 40 meters running speed obtained an average = 6.8730 with a standard deviation = 0.33106. The analysis results obtained tcount = 9,487> ttable (19: 0,025) = 2,093, with Sig. (2-tailed) = 0,000; it turns out that Sig. (2-tailed) <0.05 thus the tcount is significant. The conclusion is that there is an influence of interval training on the running speed of 40 meters on Tebing Siring 3 Elementary School students in Tanah Laut Regency.