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The purpose of this research is to determine the the best media form different seeds media for the growth of pink oyster mushroom mycelium (Pleurotus flabellatus) and the secondary metabolite composition as anti-tumor drugs. The method used was the experimental research method, one factorial Completely Randomized Design with four treatments on different seeds sources in solid and liquid media. To determine the best media we compare the weight of biomass and the secondary metabolites composition. The results showed that the growth of corn media diameter had the fastest growth rate of 86% and 82% control, 61% significantly different in soybeans and 63% in green beans. Biomass results show that the most effective media to obtain biomass is that the green beans have a weight of 26 mg and corn 25.6 mg, while potatoes are 14.7 mg and soybeans 5.9 mg. All carbohydrates media can be used as a medium for the growth of pink oyster mushroom mycelium. The secondary metabolites detected were alkaloids and saponins, while steroids and tannins were not detected in all samples, so pink oyster mushrooms have the potential to have antitumor content.