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Background. Garcinia latissima Miq. (Clusiaceae) grows on the island of Seram Maluku and Papua, but was cultivated in the Bogor Botanical Garden since May 11th 1994. Previous research has been done that ethyl acetate extract of G. latissima Miq. leaves with 2% concentration in dimethyl sulfoxide against Bacillus subtilis used agar diffusion method gave inhibition zone diameter 7.68 mm. The minimum inhibitory concentration (MIC) of the extract against B. subtilis was 5,000 ppm and the minimum bactericidal concentration (MBC) was 10,000 ppm. Purpose. The purpose of the study was to get isolate from G. latissima Miq. leaves ethyl acetate extract and to understand its activity against B. subtilis. Methods. The isolation was performed by open column chromatography, recrystallization, preparative thin layer chromatography and the isolate antibacterial assay by microdilution method. Spectroscopic characterization using ultra violet spectroscopy (UV-Vis), infra red spectroscopy (FTIR), 1H-NMR, 13C-NMR, DEPT, HSQC. Result. The isolated compound was white crystal, soluble in chloroform and insoluble in n-hexane, ethyl acetate. The melting point of the isolated compound was 262-264oC. IR spectrum showed strong signals for C-H stretching of methyl groups and carbonyloxygen (C=O). The based on NMR data, the chemical shifts of chemical compounds from the isolate was compared with the literature showed that the isolate was friedelin. The MIC of friedelin against B. subtilis was 2,500 ppm.