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Myoglobin is a cytoplasmic hemoprotein with an important role of maintaining oxygen homeostasis of tissues. The brain needs quite a high amount of oxygen to carry out its metabolism. Therefore, this study was conducted to detect the expression level of myoglobin mRNA in the brain of normal rats (Rattus novergicus).The study was conducted at the Biochemistry and Molecular Biology Laboratory of the Biology Major of the Faculty of Mathematics and Natural Sciences, UNJ from December 2010 to February 2011. Through 1.5% agarose gel electrophoresis, results show that the myoglobin band of 114bp was detected in the brain of normal rats (Rattus novergicus). Due to the positive results, there is a huge possibility that the myoglobin expressed in the brain of rats (Rattus novergicus) would be altered into protein during environmental conditions of low oxygen concentration.