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Sago is a plant that is widely cultivated in Indonesia with a planting area of 1.128 million ha. Sago waste, especially sago pulp, has quite a lot of starch so that it can be used as an amylolytic bacteria substrate. Amylase enzyme that produced by bacteria have the potential to be applied in industrial sector, food, medicine, and also be used as an effort to utilize waste. This study aims to obtain amylolytic bacteria isolated from the sago pulp industry in Palopo, South Sulawesi and the ability to produce amylase enzyme. Samples were taken from sago pulp of traditional sago industry in Palopo, South Sulawesi and isolated by using selective media. Pure isolate then screened and observed the ability to produce amylase enzyme. The results were obtained 9 potential isolates with qualitative assay obtained the highest amylolytic index shown by isolates 2B (9.004mm), 2D (7.28mm), and 4A (4.67mm). Quantitative assay obtained the highest amylase enzyme activity test shown by isolate 2B (3.62 U/mL) indicated that isolates from sago pulp had good potential to produce amylase activity.