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For the purpose of neutron imaging, scintillation crystals composed of Li, B or Gd, which have very high thermal neutron absorption coefficients are becoming popular. These crystals can provide distinct luminescence properties with different rare earths dopants (Ce3+, Dy3+, Eu3+ etc.). In the present work, Li6Y(BO3)3 (LYBO) pure and doped with 4 mol% Dy3+ single crystals have been grown using the Czochralski method. X-ray induced luminescence properties have been studied for both pure and doped crystal at room temperature. Thermoluminescence properties of both pure and 4 mol% Dy3+ doped LYBO crystals have been evaluated at low temperature from 10 K to 320 K and at high temperature from 326 K to 670 K separately. Photoluminescence, decay time and beta scintillation counts have been measured in low temperature from 290 K to 10 K for 4 mol% Dy3+ doped LYBO crystal. Characteristic emission of Dy3+ ion has been observed for LYBO crystal doped with 4 mol% Dy3+ under X-ray irradiation. The maximum intensity of excitation and emission peaks are found at 350 nm and 578 nm, respectively. Decay time decreases from 690 μm at 290 K to 655 μm at 10 K. Thermoluminescence emission of both pure and doped crystal is similar at low temperature but differs at high temperature. Scintillation beta counts has also been decreased while measuring from 320 K to 10 K. In future, the crystal will be tested as a thermal neutron scintillator for imaging.