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Environmental factors influences the composition of plant metabolites, both qualitatively and quantitatively. Plants pharmacological activity depends on its metabolites composition. Dapdap (Erythrina variegata L.) is one of the plants that is part of Balis local wisdom. Dapdap is easily found in the tropics and can grow on various textures and soil pH. Dapdap and turmeric are ingredient of "tepung tawar", which is a means of spiritual purity in Bali-s religious ceremonies. The application of local wisdom values is supported by the results of the antibacterial activity test of dapdap extract against pathogenic bacteria. This study was to determine the antibacterial activity of dapdap extract obtained from different sampling locations. The well diffusion method was used to evaluate the antibacterial activity of dapdap extract against Staphylococcus aureus and Eschericia coli. The extract was obtained by maceration for 24-72 hours using 96% ethanol. The addition of turmeric extract was able to increase the antibacterial activity of dapdap extract. The different of sampling location can affect the antibacterial activity against Eschericia coli. The maceration duration of dapdap extract did not affect its antibacterial activity against Staphylococcus aureus and Eschericia coli.