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Domestic waste produced by hospitals can reach 85% of total waste with high pollutant content. The high content of these pollutants can have an impact on human health as well as the environment. This study aims to detect the presence of the α-Amylase enzyme by gene code toward Enterobacter agglomerans. E. agglomerans is a bacterium that lives in waste and is able to degrade starch by hydrolyzing α-1.4 glucosidic amylose, amylopectin, and glycogen bonds. The study was conducted in December 2018 to March 2019 in the Microbiology Laboratory of Universitas Negeri Malang for the preparation of E. agglomerans and in the Microbiology Laboratory of Universitas Brawijaya to isolate DNA and detect the α-Amylase gene. The results showed that bacterial DNA isolation was of good quality with a purity value of 1.87 nm and a DNA concentration of 204.02 μg / ml. The α-Amylase gene in E. agglomerans was detected at 46oC, 48oC and 50oC. These results indicate that E. agglomerans has a gene that encodes the α-Amylase enzyme so that it can be used as a bioremediator candidate.